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用於生產一標的蛋白質的核酸建構物、重組型載體以及方法
Disclosed herein is a process for producing a target protein, in which a recombinant polynucleotide is constructed to encode a fusion protein including: (i) an anchoring protein that includes a N-terminal amino acid sequence of an ice nucleation protein, so that the fusion protein, once expressed in the host cell, is directed by the anchoring protein to be anchored and exposed on the outer membrane of the host cell; (ii) the target protein; and (iii) a self-splicing protein that includes a first end fused with the anchoring protein and a second end fused with the target protein, wherein the self-splicing protein includes a N-terminal or C-terminal amino acid sequence of an intein protein at the second end thereof, such that upon an environmental stimulus, the self-splicing protein exerts a self-cleavage at the second end thereof to release the target protein from the fusion protein.
閱讀詳細內容固定化金屬親和薄膜及其製備方法、由其製得之金屬錯合膜以及固定化酵素膜
本發明固定化金屬親和薄膜之製備方法包含步驟為：令一表面具有羥基之薄膜與一鹼液於一相轉移催化劑存在下進行反應，以取得一活化膜；在一兩性溶劑的存在下，令該活化膜與一環氧化物相反應，使該活化膜的表面具有多個環氧基；以及令該具有多個環氧基之活化膜與一含有螯合劑且pH值範圍介於10~14之間的水溶液相反應，進而製得一表面具有多個可螯合金屬離子的螯合基之固定化金屬親和薄膜。由上述之方法所製得之固定化金屬親和薄膜可螯合金屬離子的量是介於4.0~4.6 mmole/cm2之間。本發明還提供該親和薄膜螯合金屬離子後製得之金
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