技術內容
脂肪酶 (lipase) 是普遍存在於生物界之一種酵素，包括微生物、植物及動物界。藉由飼料中添加適量微生物亦能提升其它受質之消化及吸收。動物飼料添加酵素，在飼料加工造粒時有一高溫 (80-90°C) 處理過程。若酵素可耐高溫及活性可穩定維持，可提高利用性及商業化價值。以原核表達系統表現定點基因突變之重組 lipase 蛋白，經 SDS-PAGE 及以西方墨點法 (Western blot) 確認重組蛋白。此lipase具耐熱性及酸鹼 (25-90°C 及 pH 4-10 皆具活性)。
Lipase is an enzyme commonly found in the biosphere. There are a variety of enzymes which is derived from microorganisms including fungi and bacteria. To make the addition of the enzymes to animal feed, it must be processed in a high temperature (80-90°C). If the enzyme activity can be stably maintained in the process, its utilization and commericalized value will be increased. The gene of lipase was point mutated and expressed in E. coli expression system and the expressed protein was examined by SDS-PAGE and Western blot assays. We found that the lipase is heat- and acid-resistant.
廠商資格
1、廠商業別：生物技術&飼料廠
2、應具備之專門技術：發酵槽及及蛋白技術
3、應有之機具設備：發酵槽
4、應有之研究或技術人員人數：5
5、其他：無
預期利用範圍
耐高溫 (80-90°C) 之脂肪酶添加小豬飼料, 使豬隻毛色較光澤及促進豬之生長及增重。也可應用於家禽。